It can be crucial that you don't shop your HPLC column when it nonetheless has any buffers or ion-pairing agents. To ensure this, flush five column quantity Distorted or doublet peak shapes: Evaluate your peaks. Doublet peaks outcome from the blockage for the frit of the column, leading to el

, inspecting the tubes at three hours and subsequently at suited intervals as many as 24 hours. Test constructive and destructive controls at the same time Along with the unfamiliar specimens. If no coagulation in almost any diploma is noticed, the specimen satisfies the requirements of your test fo